Knowledge Hub
Guides on quality verification, reconstitution, and safe handling of research peptides. For laboratory research use only.
Cell-free versus cell-based peptide assays: choosing a model for the question
Cell-free peptide assays omit membranes and intracellular fate. Compare access, metabolism and endpoints, and why a chemical hit may not transfer.
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SPR versus fluorescence assays for peptide binding: different readouts, different artifacts
Four 2026 abstracts cover fluorescence peptide binding, not SPR. Compare labeling, apparent Kd and membrane artifacts before choosing orthogonal tests.
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Linear versus cyclic peptides: stability, conformation and target access
Matched linear–cyclic pairs can diverge on Aβ42, MALAT1 and radiation; unmatched RGD work is not the same comparison.
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Binding Affinity Versus Functional Potency: Comparing Peptide Evidence Fairly
Picomolar KD is not cell potency. Four 2026 peptide abstracts show how occupancy and response differ—and which cross-paper ranks fail.
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IC50 versus EC50: Why Peptide Assay Numbers Are Not Interchangeable
IC50 marks 50% inhibition; EC50 marks 50% of a defined response. Peptide papers often report both—here is why those numbers cannot be swapped.
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